[目的]为了更好的了解泡泡刺黄酮类生物合成途径及其潜在的抗逆作用,[方法]应用高通量RNA-seq测序技术对泡泡刺当年生新叶进行了转录组测序及相关生物信息学分析。[结果]表明:泡泡刺当年生叶中获得了13 013 444 total reads,总核苷酸数为1 171 209 960 bp(1.09 Gb),组装拼接后得到48 921条unigene序列;Nr、Swiss-Prot、COG、GO、KEGG等数据库分析显示,有30 407个Nr注释,19 671个Swissprot注释,9 273个COG功能注释,46 153个GO功能注释,13 654个KEGG注释,并从KEGG通路中找到参与黄酮类化合物合成途径的关键基因片段186个。[结论]本研究获得了较好的泡泡刺转录组序列信息,并且其中富含黄酮类化合物代谢途径的相关基因,这为其抗逆机制、药用价值的研究及开发利用提供宝贵资源。

目的 探究林龄对华北落叶松林枯落物水文效应的影响。 方法 于2017年6月在宁夏六盘山香水河小流域选择4种林龄阶段（16、25、34、43a）的华北落叶松人工林样地，调查林分结构和测量林下枯落物蓄积量、厚度、持水量等指标，分析不同林龄华北落叶松枯落物层持水能力差异。 结果 研究表明：（1）华北落叶松枯落物厚度介于4.5~6.0 cm，总蓄积量在29.08~33.21 t·hm^-2，且半分解层蓄积量高于未分解层蓄积量，4种林龄枯落物厚度与蓄积量均表现为成熟林>近熟林>中龄林>幼龄林。（2）各龄林枯落物最大持水量介于79.47~110.05 t·hm^-2，成熟林最大；最大持水率变动在273.32%~341.27%，中龄林最大。（3）各龄林枯落物持水量、吸水速率与浸水时间动态变化均类似，枯落物持水过程表现为浸水0.5 h内吸水速率最大，4 h之后吸水速率趋于平缓，10 h后枯落物持水量基本饱和，持水量与浸水时间均呈明显对数关系（R²>0.92）。（4）各龄林枯落物有效拦蓄量在43.64~70.52 t·hm^-2之间，成熟林拦蓄能力最强。 结论 综合分析4种林龄枯落物水文效应，成熟林枯落物层水文功能最强。

The results of seedling stage trial showed that with the pattern of sowing seeds as they are collected, transplanting in the row spacing of 15 cm×20 cm, shading and under normal cultivation conditions, the mean height and basal diameter of 16 month old seedlings were 72.9 cm and 0.98 cm,respectively. The survival rate of afforestation of bare rooted seedlings reached as high as 98.5%. The increment peak of tree height and DBH is in May August. The growth period is in April September and the stagnant period of growth is in January March and October December. At the age of 4 years old, the current annual increments of height and DBH were the maximum. The mixed stand of M. hedyosperma and Cunninghamia lanceolata has well stand structure. Under the optimal mixing treatment, the mean tree height and DBH of 4 year old M. hedyosperma are 3.99 m and 6.18 cm, while that of C. lanceolata are 5.05 m and 8.55 cm, which is 10.1% and 19.1% higher than that of C. lanceolata pure stand.